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KMID : 0357319950300030345
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Journal of the Korean Society for Microbiology
1995 Volume.30 No. 3 p.345 ~ p.358
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Modulated Expression of Cell Envelope and SOD Activity of Klebsiella Pneumoniae in Response to Growth Condition
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이상화
김정민/우영훈/이제철/이유철/김정완/설성용/조동택
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Abstract
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Clinical isolates of six strains of Klebsiella pneumoniae that were grown under various conditions showed modulation of expression in envelope proteins. Outer membrane protein (OMR) profiles obtained by SDS-PAGE were significantly different by
each
of
three different culture media; iron supplemented (Fe+SSM) or removed (Fe+SSM) simple salt media and LB medium as control. All strains grown in Fe+SSM showed prominent expression of, at least 5, iron regulated OMPs in various molecular size
ranging
70 to
83 kilodalton.
Lipopolysaccharide 9LPS) profiles of K. pneumoniae was composed of typical stepladder shaped O-polysaccharide and densely stained core-glycolipid. Presence or absence of iron in culture media did not affect LPS profiles but there was only a
length
diversity of O-polysaccharide by culture media.
Both of FeSOD were detected in the strains grown in LB medium by native PAGE. Expression of FeSOD was greater than MnSOD when cultured in Fe+SSM, on the contrary, MnSOD was greater than FeSOD when grown in FeSSM.
SOD activity assayed by the epinephrine autoxidation of strains grown in Fe+SSM was higher than that of strains grown in Fe-SSM or LB medium. Sputum isolates showed lower SOD ativity than that of urine of blood isolates. These data suggested more
potent
ability of FeSOD than that of MnSOD to scavenge reactive oxygen radical.
The ability of K. pneumoniae to survive within macrophage was variable by each strain. There was no common feature between origin of strains or kind of medium used. No correlation was observed between survival within macrophage and SOD production
of K.
pneumoniae. Other potential mechanisms might be involved in the survival of K. pneumoniae in macrophage than the ability to produce SOD.
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